Issue 12, 2012

Synthesis, characterization of a novel phenyleneethynylene derivative and application in a fluorescence DNA sensor

Abstract

In this paper, a novel phenyleneethynylene derivative 4,4′-(2,5-dimethoxy-1,4-phenylene)bis(ethyne-2,1-diyl) dibenzoic acid (p-acid) was synthesized. Infrared spectroscopy (IR), nuclear magnetic resonance (NMR), photoluminescence (PL) spectroscopy and ultraviolet visible (UV-vis) spectroscopy were applied to characterize p-acid. The p-acid doped silica nanoparticle (p-acid@SiO2) was assembled in layer-by-layer (LBL) self-assembling processes using tetraethoxysilane (TEOS). Scanning electron microscope (SEM) and transmission electron microscope (TEM) were applied to characterize p-acid@SiO2. To research the practical applicability, a sample DNA sensor was constructed using p-acid@SiO2 label, in which the PL intensity response was proportion to the target-DNA (S2) concentration in the range of 0.05–1000 fM, with a detection limit of 20 pM. Furthermore, the DNA sensor showed high specificity, excellent stability, and good reproducibility. The p-acid@SiO2-based DNA sensor can also provide potential application for detection of other pathogen DNA.

Graphical abstract: Synthesis, characterization of a novel phenyleneethynylene derivative and application in a fluorescence DNA sensor

Article information

Article type
Paper
Submitted
24 Sep 2012
Accepted
24 Oct 2012
First published
24 Oct 2012

Anal. Methods, 2012,4, 4339-4345

Synthesis, characterization of a novel phenyleneethynylene derivative and application in a fluorescence DNA sensor

C. Chu, X. Wang, S. Li, S. Ge, L. Ge, J. Yu and M. Yan, Anal. Methods, 2012, 4, 4339 DOI: 10.1039/C2AY26086G

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