Simultaneous determination of four nucleosides in bee pollens of various floral origin by HPLC-UV detector

Abstract

An improved HPLC method was developed for the simultaneous determination of nucleosides in bee pollen samples of various floral origins. Bee pollen samples were dissolved in 35 mL pure water by agitation on a vortex mixer followed by ultrasonic-assisted extraction prior to quantitative analysis. HPLC conditions were optimized and good linearity (r² > 0.999) was obtained over the investigated concentration ranges. Precision was evaluated by intra- and inter-day assays and RSD values were below 3.15%. Accuracies ranged from 82.7% to 124.7%, respectively. Finally, the method was successfully applied to the analysis of nucleosides in bee pollen samples and could be used for routine analysis as the standard method.