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Issue 10, 2012
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Extract-free immunochromatographic assay for on-site tests of aflatoxin M1 in milk

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Abstract

Lateral flow immunochromatographic strip tests (IST) are very promising for use as on-site test diagnostic tools in developing countries. In this work, a fabrication process simplified, lateral flow immunochromatographic test strip for aflatoxin M1 (AFM1) detection in milk samples has been developed. The immunostrip was specific to AFM1 showing no cross-reactivity with other major aflatoxins. The visual detection limit (VDL) and threshold level for AFM1 was 0.3 and 4.0 ng mL−1, respectively. Evaluation results of the IST revealed good accuracy and repeatability. In the detection of AFM1 contamination level in milk samples, visual results of the IST are in good correlation with those of a quantitative enzyme-linked immunosorbent assay (ELISA). This AFM1–IST, employing a one-step method, was environmentally friendly without the use of any organic reagents or buffers and additional equipment, and could serve as an on-site and home-use device for AFM1 tests in milk, and be helpful to people's health care.

Graphical abstract: Extract-free immunochromatographic assay for on-site tests of aflatoxin M1 in milk

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Article information


Submitted
27 Feb 2012
Accepted
12 Jun 2012
First published
13 Jun 2012

Anal. Methods, 2012,4, 3307-3313
Article type
Paper

Extract-free immunochromatographic assay for on-site tests of aflatoxin M1 in milk

D. H. Zhang, P. W. Li, Q. Zhang, Y. Yang, W. Zhang, D. Guan and X. X. Ding, Anal. Methods, 2012, 4, 3307
DOI: 10.1039/C2AY25205H

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