Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 6, 2012
Previous Article Next Article

Statistical analysis of discrete encapsulation of nanomaterials in colloidal capsules

Author affiliations

Abstract

Encapsulation of materials into capsules becomes discrete at small scales. We propose a measurement and analysis method of discrete encapsulation of nanomaterials in colloidal capsules, with the volume of fL to pL, using the fluorescence flow cytometry. Encapsulation of nano-sized polymer beads and lambda-phage DNA into lipid vesicles (liposomes) is evaluated as a model system. By using fluorescence markers, vesicle volume, membrane quantity and nanomaterials in a large number of individual vesicles are measured simultaneously. We show that the number of encapsulated material (k = 0, 1, 2, …) can be evaluated from the quantized fluorescence intensity distribution, which is then compared with the Poisson statistics. As a result, probability of encapsulating beads and λDNA exhibits characteristic trends that depend on the vesicle volume, lamellarity, and electrostatic interaction, which should reflect the physical interaction in the vesicle formation processes. This measurement and analysis will be a powerful tool in designing the colloidal capsules used in many scientific and industrial applications.

Graphical abstract: Statistical analysis of discrete encapsulation of nanomaterials in colloidal capsules

Back to tab navigation

Supplementary files

Article information


Submitted
31 Jan 2012
Accepted
06 Apr 2012
First published
10 Apr 2012

Anal. Methods, 2012,4, 1648-1655
Article type
Paper

Statistical analysis of discrete encapsulation of nanomaterials in colloidal capsules

T. Sakakura, K. Nishimura, H. Suzuki and T. Yomo, Anal. Methods, 2012, 4, 1648
DOI: 10.1039/C2AY25105A

Social activity

Search articles by author

Spotlight

Advertisements