Jump to main content
Jump to site search

Issue 7, 2012
Previous Article Next Article

Determination of eight tobacco alkaloids in flue-cured tobacco samples by gas chromatography with nitrogenchemiluminescence detection (NCD)

Author affiliations

Abstract

A reliable, sensitive and rapid method for determination of eight alkaloids in flue-cured tobacco by dual-column-dual-detector gas chromatography with nitrogen chemiluminescence detection (GC-NCD) has been established. At first, the extraction effects of several factors such as solid to liquid ratio, extraction time, sodium hydroxide solution volume and sodium hydroxide solution mass fraction on the extraction efficiency were simultaneously investigated using an orthogonal array design (OAD). Then, the NCD was compared to other detectors and showed some unique advantages. The analytical method met the strict quantitative requirement by optimizing chromatographic conditions and using dual-column confirmation. Finally, the performance of the proposed method was tested for the extraction and determination of the eight alkaloids in flue-cured tobacco and satisfactory results were achieved.

Graphical abstract: Determination of eight tobacco alkaloids in flue-cured tobacco samples by gas chromatography with nitrogen chemiluminescence detection (NCD)

Back to tab navigation

Article information


Submitted
18 Jan 2012
Accepted
12 Apr 2012
First published
12 Apr 2012

Anal. Methods, 2012,4, 2095-2100
Article type
Paper

Determination of eight tobacco alkaloids in flue-cured tobacco samples by gas chromatography with nitrogen chemiluminescence detection (NCD)

K. Cai, Z. Xiang, J. Zhang, S. Zhou, Y. Feng and Z. Geng, Anal. Methods, 2012, 4, 2095
DOI: 10.1039/C2AY25067E

Social activity

Search articles by author

Spotlight

Advertisements