Comparison study on nucleosides and nucleotides in edible mushroom species by capillary zone electrophoresis

Abstract

A capillary zone electrophoresis (CZE) method was developed for the simultaneous determination of four nucleosides (adenosine, guanosine, uridine and inosine) and three nucleotides (guanosine-5′-monophosphate (GMP), adenosine-5′-monophosphate (AMP) and uridine-5′-monophosphate (UMP)) in nine edible mushrooms including Lasiosphaera Seu Calvatia (puffball), Agrocybe aegerita, Boletus nigricans, Boletus fulvus, Tricholoma matsutake, edible tree fungus (Auricularia auricula), Tuckahoe (Poria cocos), white fungus (Tremella fuciformis) and Polyporus umbellatus. The results indicated that some of the tested mushroom species, such as tuckahoe, white fungus and Polyporus umbellatus, had no detectable nucleosides and nucleotides. Furthermore, only the nucleotide GMP can be detected in very small amounts in puffball and Boletus fulvus. In addition, puffball has a greater amount of total nucleosides as compared to other tested samples.