Issue 24, 2012
From the journal:

Analyst

A peroxidase-active aptazyme as an isothermally amplifiable label in an aptazyme-linked oligonucleotide assay for low-picomolar IgE detection†

Jeroen Pollet,a   Ulrich Strychb  and  Richard C. Willson*ac  

* Corresponding authors

a Department of Chemical & Biomolecular Engineering, University of Houston, TX 77204, USA

b Department of Biology and Biochemistry, University of Houston, TX 77204, USA

c The Methodist Hospital Research Institute, Houston, TX 77030, USA
E-mail: willson@uh.edu
Tel: +1 713-743-4300

Abstract

A peroxidase-active hemin-aptamer was clicked onto an immunoglobulin E (IgE) binding aptamer. The resulting bifunctional aptamer was successfully used in a highly sensitive aptazyme-linked oligonucleotide assay (ALONA) for the detection of IgE. The sensitivity of the assay is enhanced by isothermal amplification of the aptazyme followed by colorimetric detection of the aptazyme's peroxidase activity, which can easily be evaluated even without advanced lab instruments.

Graphical abstract: A peroxidase-active aptazyme as an isothermally amplifiable label in an aptazyme-linked oligonucleotide assay for low-picomolar IgE detection

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Article information

DOI
https://doi.org/10.1039/C2AN36201E
Article type
Communication
Submitted
26 Aug 2012
Accepted
21 Oct 2012
First published
23 Oct 2012

Analyst, 2012,137, 5710-5712

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A peroxidase-active aptazyme as an isothermally amplifiable label in an aptazyme-linked oligonucleotide assay for low-picomolar IgE detection

J. Pollet, U. Strych and R. C. Willson, Analyst, 2012, 137, 5710 DOI: 10.1039/C2AN36201E

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