Issue 19, 2012
From the journal:

Analyst

Dispersive liquid–liquid microextraction using functionalized Mg(OH)2 NPs with oleic acid as hydrophobic affinity probes for the analysis of hydrophobic proteins in bacteria by MALDI MS†

Suresh Kumar Kailasaabc  and  Hui-Fen Wu*abd  

* Corresponding authors

a Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan
E-mail: hwu@faculty.nsysu.edu.tw
Fax: +886-7-5253908
Tel: +886-7-5252000-3955

b Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung, Taiwan

c Department of Applied Chemistry, S. V. National Institute of Technology, Surat – 395007, India

d Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan

Abstract

We report a dispersive liquid–liquid microextraction (LLME) method using surface modified Mg(OH)2 nanoparticles (NPs) with oleic acid (OA) as hydrophobic affinity probes for the extraction and preconcentration of hydrophobic biomolecules prior to MALDI MS analysis. OA-capped Mg(OH)2 NPs were characterized by FT-IR, SEM and TEM. The functionalized Mg(OH)2 NPs are well dispersed in toluene with an average diameter of <35 nm. Under the optimum conditions, the method showed good linearity in the ranges of 10–400 nM and 0. 1–2.0 μM for valinomycin and gramicidin D, respectively. The limits of detection were in the region of 2.0 nM and 24 nM for valinomycin and gramicidin D, respectively. Moreover, the OA-capped Mg(OH)2 NPs-assisted LLME method was successfully used for the rapid extraction/preconcentration of hydrophobic proteins in bacteria (Escherichia coli and Bacillus subtilis) prior to their identification by MALDI MS.

Graphical abstract: Dispersive liquid–liquid microextraction using functionalized Mg(OH)2 NPs with oleic acid as hydrophobic affinity probes for the analysis of hydrophobic proteins in bacteria by MALDI MS

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Article information

DOI
https://doi.org/10.1039/C2AN35788G
Article type
Paper
Submitted
12 Jun 2012
Accepted
07 Jul 2012
First published
09 Jul 2012

Analyst, 2012,137, 4490-4496

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Dispersive liquid–liquid microextraction using functionalized Mg(OH)2 NPs with oleic acid as hydrophobic affinity probes for the analysis of hydrophobic proteins in bacteria by MALDI MS

S. K. Kailasa and H. Wu, Analyst, 2012, 137, 4490 DOI: 10.1039/C2AN35788G

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