Demonstration of a plasmonic thermocycler for the amplification of human androgen receptor DNA

Philip J. R. Roche; Lenore K. Beitel; Rifat Khan; Rose Lumbroso; Mohamed Najih; Maurice C.-K. Cheung; Joachim Thiemann; Venkat Veerasubramanian; Mark Trifiro; Vamsy P. Chodavarapu; Andrew G. Kirk

doi:10.1039/C2AN35692A

Demonstration of a plasmonic thermocycler for the amplification of human androgen receptor DNA

Philip J. R. Roche,*^a Lenore K. Beitel,^bc Rifat Khan,^d Rose Lumbroso,^b Mohamed Najih,^a Maurice C.-K. Cheung,^d Joachim Thiemann,^e Venkat Veerasubramanian,^a Mark Trifiro,^bc Vamsy P. Chodavarapu^d and Andrew G. Kirk^a

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^a The Photonic Systems Group, McGill University, 3480 University St Room 753, Montreal, Quebec, Canada H3A 2A7
E-mail: philjrroche@hotmail.com

^b Lady Davis Institute for Medical Research, Jewish General Hospital, 3755 Côte Ste-Catherine Road, Montreal, Quebec, Canada

^c Faculty of Medicine, McGill University, Montreal, Quebec, Canada H3G 1Y6

^d McGill University, Department of Electrical and Computer Engineering, Room 642, 3480 University Street, Montreal, Quebec, Canada H3A 2A7

^e Telecommunication & Signal Processing Lab, Multimedia Signal Processing, Dept. of Electrical and Computer Engineering, McGill University, Montreal, Quebec, Canada H3A 2A7

Abstract

A plasmonic heating method for the polymerase chain reaction is demonstrated by the amplification of a section of the human androgen receptor gene. The thermocycler has a simple low-cost design, demonstrates excellent temperature stability and represents the first practical demonstration of plasmonic thermocycling.

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Demonstration of a plasmonic thermocycler for the amplification of human androgen receptor DNA

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