Jump to main content
Jump to site search

Issue 16, 2012
Previous Article Next Article

Rapid and simple preparation of a reagentless glucose electrochemical biosensor

Author affiliations

Abstract

A rapid and simple procedure was developed for the preparation of a highly stable and leach-proof glucose oxidase (GOx)-bound glassy carbon electrode (GCE). Crosslinked GOx via glutaraldehyde was drop-cast on a KOH-pretreated GCE followed by drop-casting of 3-aminopropyltriethoxysilane (APTES) to form a stable bioactive layer. At −0.45 V, the biosensor exhibited a wide dynamic detection range of 0.5–48 mM for commercial glucose and 1.3–28.2 mM for Sugar-Chex blood glucose linearity standards. Several endogenous electroactive substances and drug metabolites commonly found in blood were tested and provoked no signal response. To our knowledge, the developed procedure is the most rapid method for preparing a glucose biosensor. The biosensor suffered no biofouling after 7 days of immersion in Sugar-Chex blood glucose. With excellent production reproducibility, GOx-bound electrodes stored dry at room temperature retained their initial activity after several weeks.

Graphical abstract: Rapid and simple preparation of a reagentless glucose electrochemical biosensor

Back to tab navigation

Supplementary files

Article information


Submitted
28 Jan 2012
Accepted
05 Jun 2012
First published
07 Jun 2012

Analyst, 2012,137, 3800-3805
Article type
Paper

Rapid and simple preparation of a reagentless glucose electrochemical biosensor

D. Zheng, S. K. Vashist, K. Al-Rubeaan, J. H. T. Luong and F. Sheu, Analyst, 2012, 137, 3800
DOI: 10.1039/C2AN35128E

Social activity

Search articles by author

Spotlight

Advertisements