Issue 6, 2012
From the journal:

Analyst

Negative visualization of DNA in agarose gel by eosin Y and its related mechanism

Weitao Cong,†a   Weijing You,†a   Mao Chen,a   Jin Ling,a   Zhongxin Zhu,a   Jungkap Choi,b   Litai Jin*acd  and  Xiaokun Lia  

* Corresponding authors

a Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College, Wenzhou, Zhejiang, P. R. China
E-mail: jin_litai@yahoo.cn
Fax: +86-577-86699790
Tel: +86-577-86699790

b College of Pharmacy & Research Institute of Drug Development, Chonnam National University, Gwangju, South Korea

c School of Basic Medical Sciences, Jilin University, Changchun, Jilin, China

d Wenzhou Undersun Biotechnology Co., Ltd., Wenzhou, Zhejiang, China

Abstract

We report here a sensitive and environmentally benign technique for the visualization of DNA by using eosin Y, which appeared as a transparent and colorless band under the opaque gel matrix background. As low as 0.2–0.6 ng of DNA can be visualized within 1 h, similar to that of the most commonly used ethidium bromide stain. Furthermore, the mechanism of the newly developed method was also described. According to the results, it can be concluded that the complex between DNA and eosin Y is mainly formed by the function of groove binding, but not intercalate itself into the double helix structure of DNA.

Graphical abstract: Negative visualization of DNA in agarose gel by eosin Y and its related mechanism

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/C2AN00008C
Article type
Paper
Submitted
02 Jan 2012
Accepted
12 Jan 2012
First published
13 Jan 2012

Analyst, 2012,137, 1466-1472

Permissions

Request permissions

Negative visualization of DNA in agarose gel by eosin Y and its related mechanism

W. Cong, W. You, M. Chen, J. Ling, Z. Zhu, J. Choi, L. Jin and X. Li, Analyst, 2012, 137, 1466 DOI: 10.1039/C2AN00008C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements