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Issue 1, 2012

Micromechanical measurement of AChBP binding for label-free drug discovery

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Abstract

A potential binding assay based on binding-driven micromechanical motion is described. Acetylcholine binding protein (AChBP) was used to modify a microcantilever. The modified microcantilever was found to bend on application of the naturally occurring agonist (acetylcholine) or the antagonist (nicotine and d-tubocurarine). Control experiments show that microcantilevers modified without AChBP do not respond to acetylcholine, nicotine, and d-tubocurarine. Kd values obtained for acetylcholine, nicotine, and d-tubocurarine are similar to those obtained from radio-ligand binding assays. These results suggest that the microcantilever system has potential for use in label free, drug screening applications.

Graphical abstract: Micromechanical measurement of AChBP binding for label-free drug discovery

Article information


Submitted
10 Aug 2011
Accepted
29 Sep 2011
First published
02 Nov 2011

Analyst, 2012,137, 263-268
Article type
Paper

Micromechanical measurement of AChBP binding for label-free drug discovery

K. Buchapudi, X. Xu, Y. Ataian, H. Ji and M. Schulte, Analyst, 2012, 137, 263 DOI: 10.1039/C1AN15734E

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