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Issue 13, 2011
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Size-dependent detachment of DNA molecules from liquid–liquid interfaces†

Thomas Hahna  and  Steffen Hardt*a  
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* Corresponding authors

a Center of Smart Interfaces, TU-Darmstadt, Petersenstrasse 32, Darmstadt, Germany
E-mail: hardt@csi.tu-darmstadt.de
Fax: +49 6151 16-72021
Tel: +49 6151 16-2874

Abstract

Binary aqueous solutions of poly(ethylene glycol) (PEG) and dextran form two phases with a liquid–liquid interface at which DNA molecules can be accumulated using an electric field. Studying DNA strands of different lengths reveals that the interface acts as a size-selective filter for molecules. Upon increasing the electric field strength, the small molecules are first detached from the interface, followed by the larger ones. Besides the contributions to a better understanding of the interaction of nanoscopic objects with liquid–liquid interfaces, the work demonstrates novel pathways for the size separation of DNA. We anticipate that by optimizing the parameters governing the detachment of molecules or nanoparticles, superior schemes for DNA separation or concepts of functional liquid interfaces could emerge.

Graphical abstract: Size-dependent detachment of DNA molecules from liquid–liquid interfaces

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Publication details

The article was received on 21 Feb 2011, accepted on 06 May 2011 and first published on 02 Jun 2011


Article type: Paper
DOI: 10.1039/C1SM05309D
Soft Matter, 2011,7, 6320-6326

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    Size-dependent detachment of DNA molecules from liquid–liquid interfaces

    T. Hahn and S. Hardt, Soft Matter, 2011, 7, 6320
    DOI: 10.1039/C1SM05309D

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