Issue 8, 2011
From the journal:

Soft Matter

Compartment size dependence of performance of polymerase chain reaction inside giant vesicles†

Koh-ichiroh Shohda,ab   Mieko Tamura,a   Yoshiyuki Kageyama,a   Kentaro Suzuki,ab   Akira Suyamaa  and  Tadashi Sugawara*ab  

* Corresponding authors

a Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo, Japan
E-mail: suga@pentacle.c.u-tokyo.ac.jp
Fax: +81-3-5454-6997
Tel: +81-3-5454-6742

b Research Center for Life Science as Complex Systems, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo, Japan

Abstract

The amplification of a 1229 bp template DNA that encoded the green fluorescent protein (GFP) was conducted in cell-sized giant vesicles (GVs, ϕ > 1 µm) using a real-time polymerase chain reaction (PCR) technique. The proportion of PCR-proceeded GV reached up to 16% of all GVs. The dependence of PCR on GV size was elucidated by flow cytometry analysis.

Graphical abstract: Compartment size dependence of performance of polymerase chain reaction inside giant vesicles

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C0SM01463J
Article type
Communication
Submitted
10 Dec 2010
Accepted
15 Feb 2011
First published
11 Mar 2011

Soft Matter, 2011,7, 3750-3753

Permissions

Request permissions

Compartment size dependence of performance of polymerase chain reaction inside giant vesicles

K. Shohda, M. Tamura, Y. Kageyama, K. Suzuki, A. Suyama and T. Sugawara, Soft Matter, 2011, 7, 3750 DOI: 10.1039/C0SM01463J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements