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Issue 5, 2011
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Glutathione (GSH)-decorated magnetic nanoparticles for binding glutathione-S-transferase (GST) fusion protein and manipulating live cells

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Abstract

Iron oxide-based magnetic nanoparticles (MNP) surface-decorated with glutathione (GSH) via a dopamine anchor bind to human α1-glutathione S-transferase (GST) with high affinity and specificity and are able to separate GST fusion proteins from cell lysates. Both the purified GST and the protein of interest (POI) preserve their innate properties. The conjugate of MNP and the GST fusion protein also enables magnetic manipulation of cells.

Graphical abstract: Glutathione (GSH)-decorated magnetic nanoparticles for binding glutathione-S-transferase (GST) fusion protein and manipulating live cells

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Publication details

The article was received on 20 Jan 2011, accepted on 17 Feb 2011 and first published on 03 Mar 2011


Article type: Edge Article
DOI: 10.1039/C1SC00030F
Citation: Chem. Sci., 2011,2, 945-948

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    Glutathione (GSH)-decorated magnetic nanoparticles for binding glutathione-S-transferase (GST) fusion protein and manipulating live cells

    Y. Pan, M. J. C. Long, X. Li, J. Shi, L. Hedstrom and B. Xu, Chem. Sci., 2011, 2, 945
    DOI: 10.1039/C1SC00030F

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