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Issue 5, 2011
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Glutathione (GSH)-decorated magnetic nanoparticles for binding glutathione-S-transferase (GST) fusion protein and manipulating live cells

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Abstract

Iron oxide-based magnetic nanoparticles (MNP) surface-decorated with glutathione (GSH) via a dopamine anchor bind to human α1-glutathione S-transferase (GST) with high affinity and specificity and are able to separate GST fusion proteins from cell lysates. Both the purified GST and the protein of interest (POI) preserve their innate properties. The conjugate of MNP and the GST fusion protein also enables magnetic manipulation of cells.

Graphical abstract: Glutathione (GSH)-decorated magnetic nanoparticles for binding glutathione-S-transferase (GST) fusion protein and manipulating live cells

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Article information


Submitted
20 Jan 2011
Accepted
17 Feb 2011
First published
03 Mar 2011

Chem. Sci., 2011,2, 945-948
Article type
Edge Article

Glutathione (GSH)-decorated magnetic nanoparticles for binding glutathione-S-transferase (GST) fusion protein and manipulating live cells

Y. Pan, M. J. C. Long, X. Li, J. Shi, L. Hedstrom and B. Xu, Chem. Sci., 2011, 2, 945
DOI: 10.1039/C1SC00030F

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