Jump to main content
Jump to site search

Issue 7, 2011
Previous Article Next Article

Functionalized nanoscale through microscale polypeptide stabilized emulsions for display of biomolecules

Author affiliations

Abstract

We have prepared stable nano- and microscale emulsion droplets using block copolypeptide surfactants that have been functionalized with biotin. We have varied the biotin density on the droplet surfaces by mixing biotinylated with non-biotinylated surfactants, and used these to optimize the level of biotinylation for binding of NeutrAvidin. Using fluorescence microscopy and dynamic light scattering we have verified specific binding of NeutrAvidin to the biotinylated droplets and developed conditions for specific binding of biotinylated ligands to the NeutrAvidin coated droplets. These experiments outline a process for preparation of a soft, nano- through microscale sized droplet platform capable of specifically conjugating and displaying biotinylated molecules.

Graphical abstract: Functionalized nanoscale through microscale polypeptide stabilized emulsions for display of biomolecules

Back to tab navigation

Supplementary files

Article information


Submitted
26 Jan 2011
Accepted
01 Mar 2011
First published
15 Mar 2011

Polym. Chem., 2011,2, 1473-1475
Article type
Communication

Functionalized nanoscale through microscale polypeptide stabilized emulsions for display of biomolecules

J. A. Hanson and T. J. Deming, Polym. Chem., 2011, 2, 1473 DOI: 10.1039/C1PY00045D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements