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Issue 7, 2011
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Block copolymer conjugates prepared by sequentially grafting from proteinsvia RAFT

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Abstract

Well-defined thermoresponsive block copolymer conjugates were prepared by two consecutive grafting-from polymerizationsvia reversible addition–fragmentation chain transfer (RAFT) polymerization. A RAFT agent containing an activated ester was conjugated to amines on lysozyme, and N-isopropylacrylamide was polymerized by grafting directly from the modified protein in aqueous buffer. Retention of the active thiocarbonylthio moieties on the ω end of the conjugated chains allowed chain extension viapolymerization of N,N-dimethylacrylamide to yield lysozyme–poly(N-isopropylacrylamide)-b-poly(N,N-dimethylacrylamide) (LYS–PNIPAM-b-PDMA) block copolymer conjugates. Analysis of the conjugates, as well as the cleaved homopolymers and block copolymers, indicated the grafting-from polymerizations were well controlled and that chain extension during block copolymerization was efficient. The temperature-responsive aqueous solution behavior of the resulting conjugates was investigated.

Graphical abstract: Block copolymer conjugates prepared by sequentially grafting from proteinsvia RAFT

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Supplementary files

Article information


Submitted
20 Jan 2011
Accepted
09 Feb 2011
First published
01 Mar 2011

Polym. Chem., 2011,2, 1531-1535
Article type
Paper

Block copolymer conjugates prepared by sequentially grafting from proteinsvia RAFT

H. Li, M. Li, X. Yu, A. P. Bapat and B. S. Sumerlin, Polym. Chem., 2011, 2, 1531
DOI: 10.1039/C1PY00031D

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