Issue 5, 2011
From the journal:

Organic & Biomolecular Chemistry

Exploring the biocatalytic scope of a bacterial flavin-containing monooxygenase†

Ana Rioz-Martínez,a   Malgorzata Kopacz,b   Gonzalo de Gonzalo,*b   Daniel E. Torres Pazmiño,b   Vicente Gotora  and  Marco W. Fraaije*b  

* Corresponding authors

a Departamento de Química Orgánica e Inorgánica, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, C/Julián Clavería 8, Oviedo, Spain

b Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen, The Netherlands
E-mail: g.de.gonzalo.calvo@rug.nl, m.w.fraaije@rug.nl
Fax: +31 50 363 4165
Tel: +31 50 363 4345

Abstract

A bacterial flavin-containing monooxygenase (FMO), fused to phosphite dehydrogenase, has been used to explore its biocatalytic potential. The bifunctional biocatalyst could be expressed in high amounts in Escherichia coli and was able to oxidize indole and indole derivatives into a variety of indigo compounds. The monooxygenase also performs the sulfoxidation of a wide range of prochiral sulfides, showing moderate to good enantioselectivities in forming chiral sulfoxides.

Graphical abstract: Exploring the biocatalytic scope of a bacterial flavin-containing monooxygenase

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Article information

DOI
https://doi.org/10.1039/C0OB00988A
Article type
Communication
Submitted
05 Nov 2010
Accepted
15 Dec 2010
First published
11 Jan 2011

Org. Biomol. Chem., 2011,9, 1337-1341

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Exploring the biocatalytic scope of a bacterial flavin-containing monooxygenase

A. Rioz-Martínez, M. Kopacz, G. de Gonzalo, D. E. Torres Pazmiño, V. Gotor and M. W. Fraaije, Org. Biomol. Chem., 2011, 9, 1337 DOI: 10.1039/C0OB00988A

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