Issue 1, 2011
From the journal:

Organic & Biomolecular Chemistry

Rapid chemical ligation of oligonucleotides by the Diels–Alder reaction†‡§

Afaf H. El-Sagheer,*ab   Vee Vee Cheongc  and  Tom Brown*a  

* Corresponding authors

a School of Chemistry, University of Southampton, Highfield, Southampton, U.K.
E-mail: ahes@soton.ac.uk, tb2@soton.ac.uk

b Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez Canal University, Suez, Egypt

c Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore

Abstract

Extremely fast and efficient Diels–Alder chemical ligation of furan and maleimide oligonucleotides has been carried out in aqueous buffer. It was possible to ligate three oligonucleotides simultaneously in a controlled manner with the aid of a complementary splint. The templated reactions proceeded within 1 min at room temperature whereas non-templated reactions were slow and incomplete. Rapid and clean methods of DNA ligation such as the one demonstrated here have potential uses in biology and nanotechnology.

Graphical abstract: Rapid chemical ligation of oligonucleotides by the Diels–Alder reaction

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Article information

DOI
https://doi.org/10.1039/C0OB00451K
Article type
Paper
Submitted
19 Jul 2010
Accepted
05 Oct 2010
First published
11 Nov 2010

Org. Biomol. Chem., 2011,9, 232-235

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Rapid chemical ligation of oligonucleotides by the Diels–Alder reaction

A. H. El-Sagheer, V. V. Cheong and T. Brown, Org. Biomol. Chem., 2011, 9, 232 DOI: 10.1039/C0OB00451K

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