Jump to main content
Jump to site search

Issue 9, 2011
Previous Article Next Article

Hydrogen peroxide sensors for cellular imaging based on horse radish peroxidase reconstituted on polymer-functionalized TiO2 nanorods

Author affiliations

Abstract

We describe the reconstitution of apo-horse radish peroxidase (apo-HRP) onto TiO2 nanorods functionalized with a multifunctional polymer. After functionalization, the horse radish peroxidase (HRP) functionalized TiO2 nanorods were well dispersible in aqueous solution, catalytically active and biocompatible, and they could be used to quantify and image H2O2 which is a harmful secondary product of cellular metabolism. The shape, size and structure of TiO2 nanorods (anatase) were analyzed by transmission electron microscopy (TEM), high resolution TEM (HRTEM), electron diffraction (ED) and X-ray diffraction (XRD). The surface functionalization, HRP reconstitution and catalytic activity were confirmed by UV-Vis, FT-IR, CLSM and atomic force microscopy (AFM). Biocompatibility and cellular internalization of active HRP reconstituted TiO2 nanorods were confirmed by a classical MTT cytotoxicity assay and confocal laser scanning microscopy (CLSM) imaging, respectively. The intracellular localization allowed H2O2 detection, imaging and quantification in HeLa cells. The polymer functionalized hybrid system creates a complete sensor including a “cell positioning system” in each single particle. The flexible synthetic concept with functionalization by post-polymerization modification allows introduction of various dyes for sensitisation at different wavelengths and introduction of various anchor groups for anchoring on different particles.

Graphical abstract: Hydrogen peroxide sensors for cellular imaging based on horse radish peroxidase reconstituted on polymer-functionalized TiO2 nanorods

Back to tab navigation

Supplementary files

Publication details

The article was received on 07 Jun 2011, accepted on 13 Jul 2011 and first published on 15 Aug 2011


Article type: Paper
DOI: 10.1039/C1NR10587F
Citation: Nanoscale, 2011,3, 3907-3914
  •   Request permissions

    Hydrogen peroxide sensors for cellular imaging based on horse radish peroxidase reconstituted on polymer-functionalized TiO2 nanorods

    M. N. Tahir, R. André, J. K. Sahoo, F. D. Jochum, P. Theato, F. Natalio, R. Berger, R. Branscheid, U. Kolb and W. Tremel, Nanoscale, 2011, 3, 3907
    DOI: 10.1039/C1NR10587F

Search articles by author

Spotlight

Advertisements