Issue 6, 2011
From the journal:

Nanoscale

In situ monitoring of single molecule binding reactions with time-lapse atomic force microscopy on functionalized DNA origami†

Na Wu,a   Xingfei Zhou,b   Daniel M. Czajkowsky,a   Ming Ye,a   Dongdong Zeng,a   Yanming Fu,a   Chunhai Fan,a   Jun Hua  and  Bin Li*a  

* Corresponding authors

a Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China
E-mail: libin@sinap.ac.cn
Fax: +86-21-59552394
Tel: +86-21-39194603

b Physical Department, Ningbo University, Ningbo Zhejiang, China

Abstract

Individual biomolecular binding events were recorded in situ by combining time-lapse atomic force microscopy and DNA origami. Single streptavidin molecules bound to specifically biotinyated DNA origami were simply counted as a function of time to obtain a direct measure of the binding rate.

Graphical abstract: In situ monitoring of single molecule binding reactions with time-lapse atomic force microscopy on functionalized DNA origami

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Article information

DOI
https://doi.org/10.1039/C1NR10181A
Article type
Communication
Submitted
17 Feb 2011
Accepted
25 Mar 2011
First published
28 Apr 2011

Nanoscale, 2011,3, 2481-2484

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In situ monitoring of single molecule binding reactions with time-lapse atomic force microscopy on functionalized DNA origami

N. Wu, X. Zhou, D. M. Czajkowsky, M. Ye, D. Zeng, Y. Fu, C. Fan, J. Hu and B. Li, Nanoscale, 2011, 3, 2481 DOI: 10.1039/C1NR10181A

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