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Issue 2, 2011
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Extracellular biosynthesis and characterization of silver nanoparticles using Aspergillus flavusNJP08: A mechanism perspective

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Abstract

The present study demonstrates an eco-friendly and low cost protocol for synthesis of silver nanoparticles using the cell-free filtrate of Aspergillus flavusNJP08 when supplied with aqueous silver (Ag+) ions. Identification of the fungal isolate was based on nuclear ribosomal DNA internal transcribed spacer (ITS) identities. Transmission electron microscopy (TEM) and energy dispersive spectroscopy (EDS) revealed the formation of spherical metallic silver nanoparticles. The average particle size calculated using Dynamic Light Scattering measurements (DLS) was found to be 17 ± 5.9 nm. UV-Visible and Fourier transform infrared (FTIR) spectroscopy confirmed the presence of extracellular proteins. SDS-PAGE profiles of the extracellular proteins showed the presence of two intense bands of 32 and 35 kDa, responsible for the synthesis and stability of silver nanoparticles, respectively. A probable mechanism behind the biosynthesis is discussed, which leads to the possibility of using the present protocol in future “nano-factories”.

Graphical abstract: Extracellular biosynthesis and characterization of silver nanoparticles using Aspergillus flavusNJP08: A mechanism perspective

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Publication details

The article was received on 07 Sep 2010, accepted on 09 Oct 2010 and first published on 18 Nov 2010


Article type: Paper
DOI: 10.1039/C0NR00656D
Citation: Nanoscale, 2011,3, 635-641

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    Extracellular biosynthesis and characterization of silver nanoparticles using Aspergillus flavusNJP08: A mechanism perspective

    N. Jain, A. Bhargava, S. Majumdar, J. C. Tarafdar and J. Panwar, Nanoscale, 2011, 3, 635
    DOI: 10.1039/C0NR00656D

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