The C2H2 zinc finger transcription factors are likely targets for Ni(II) toxicity

Ewa Kurowska; Joanna Sasin-Kurowska; Arkadiusz Bonna; Marcin Grynberg; Jarosław Poznański; Lukasz Knizewski; Krzysztof Ginalski; Wojciech Bal

doi:10.1039/C1MT00081K

The C2H2 zinc finger transcription factors are likely targets for Ni(ii) toxicity†‡

Ewa Kurowska,^a Joanna Sasin-Kurowska,^b Arkadiusz Bonna,^a Marcin Grynberg,^a Jarosław Poznański,^a Lukasz Knizewski,^b Krzysztof Ginalski^b and Wojciech Bal*^a

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^a Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland
E-mail: wbal@ibb.waw.pl
Fax: +48 22 6584636
Tel: +48 22 5922346

^b Laboratory of Bioinformatics and Systems Biology, Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw, Zwirki i Wigury 93, 02-089 Warsaw, Poland
Fax: +48 22 5540801
Tel: +48 22 5540800

Abstract

Ni(II) ions are able to hydrolyze Naa-(Ser/Thr) peptide bonds in Naa-(Ser/Thr)-Xaa-His-Zaa sequences. We found that various human transcription factors contain such nickel hydrolytic patterns within C2H2 zinc finger (ZF) domains. We demonstrated the hydrolysis on two peptide models, the 3rd ZF of the Sp1 transcription factor and the 1st ZF of the ZNF302 transcription factor. The experimentally studied reaction rates indicate that the hydrolysis reaction is likely to be an element of intracellular nickel toxicity.

This article is part of the themed collection: Metal Toxicity

Metallomics

The C2H2 zinc finger transcription factors are likely targets for Ni(ii) toxicity†‡

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