Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 4, 2011
Previous Article Next Article

An image-based 384-well high-throughput screening method for the discovery of biofilm inhibitors in Vibrio cholerae

Author affiliations

Abstract

Bacterial biofilms are assemblages of bacterial cells and extracellular matrix that result in the creation of surface-associated macrocolony formation. Most bacteria are capable of forming biofilms under suitable conditions. Biofilm formation by pathogenic bacteria on medical implant devices has been linked to implant rejection in up to 10% of cases, due to biofilm-related secondary infections. In addition, biofilm formation has been implicated in both bacterial persistence and antibiotic resistance. In this study, a method has been developed for the discovery of small molecule inhibitors of biofilm formation in Vibrio cholerae, through the use of high-throughput epifluorescence microscopy imaging. Adaptation of a strategy for the growth of bacterial biofilms in wellplates, and the subsequent quantification of biofilm coverage within these wells, provides the first example of an image-based 384-well format system for the evaluation of biofilm inhibition in V. cholerae. Application of this method to the high-throughput screening of small molecule libraries has lead to the discovery of 29 biofilm lead structures, many of which eliminate biofilm formation without altering bacterial cell viability.

Graphical abstract: An image-based 384-well high-throughput screening method for the discovery of biofilm inhibitors in Vibrio cholerae

Back to tab navigation

Supplementary files

Publication details

The article was received on 11 Nov 2010, accepted on 16 Dec 2010 and first published on 18 Jan 2011


Article type: Paper
DOI: 10.1039/C0MB00276C
Citation: Mol. BioSyst., 2011,7, 1176-1184

  •   Request permissions

    An image-based 384-well high-throughput screening method for the discovery of biofilm inhibitors in Vibrio cholerae

    K. C. Peach, W. M. Bray, N. J. Shikuma, N. C. Gassner, R. S. Lokey, F. H. Yildiz and R. G. Linington, Mol. BioSyst., 2011, 7, 1176
    DOI: 10.1039/C0MB00276C

Search articles by author

Spotlight

Advertisements