Issue 9, 2011
From the journal:

Molecular BioSystems

Photochemical control of bacterial signal processing using a light-activated erythromycin†

Laura Gardner,a   Yan Zou,a   Alexandria Mara,a   T. Ashton Cropp*b  and  Alexander Deiters*a  

* Corresponding authors

a Department of Chemistry, North Carolina State University, Raleigh
E-mail: alex_deiters@ncsu.edu
Tel: 919-515-7607

b Department of Chemistry, Virginia Commonwealth University, Richmond
E-mail: tacropp@vcu.edu
Tel: 804-828-3597

Abstract

Bacterial cells control resistance to the macrolide antibiotic erythromycin using the MphR(A) repressor protein. Erythromycin binds to MphR(A), causing release of the PmphR promoter, activating expression of the 2′-phosphotransferase Mph(A). We engineered the MphR(A)/promoter system to, in conjunction with a light-activatable derivative of erythromycin, enable photochemical activation of gene expression in E. coli. We applied this photochemical gene switch to the construction of a light-triggered logic gate, a light-controlled band-pass filter, as well as spatial and temporal control of gene expression.

Graphical abstract: Photochemical control of bacterial signal processing using a light-activated erythromycin

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Article information

DOI
https://doi.org/10.1039/C1MB05166K
Article type
Communication
Submitted
04 May 2011
Accepted
11 Jul 2011
First published
22 Jul 2011

Mol. BioSyst., 2011,7, 2554-2557

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Photochemical control of bacterial signal processing using a light-activated erythromycin

L. Gardner, Y. Zou, A. Mara, T. A. Cropp and A. Deiters, Mol. BioSyst., 2011, 7, 2554 DOI: 10.1039/C1MB05166K

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