Jump to main content
Jump to site search

Issue 23, 2011
Previous Article Next Article

Millifluidic droplet analyser for microbiology

Author affiliations

Abstract

We present a novel millifluidic droplet analyser (MDA) for precisely monitoring the dynamics of microbial populations over multiple generations in numerous (≥103) aqueous emulsion droplets (∼100 nL). As a first application, we measure the growth rate of a bacterial strain and determine the minimal inhibitory concentration (MIC) for the antibiotic cefotaxime by incubating bacteria in a fine gradient of antibiotic concentrations. The detection of cell activity is based on the automated detection of an epifluorescent signal that allows the monitoring of microbial populations up to a size of ∼106cells. We believe that this device is helpful for the study of population dynamic consequences of microbe-environment interactions and of individual cell differences. Moreover, the fluidic machine may improve clinical tests, as it simplifies, automates and miniaturizes the screening of numerous microbial populations that grow and evolve in compartments with a finely tuned composition.

Graphical abstract: Millifluidic droplet analyser for microbiology

Back to tab navigation

Supplementary files

Publication details

The article was received on 22 Jun 2011, accepted on 08 Sep 2011 and first published on 20 Oct 2011


Article type: Paper
DOI: 10.1039/C1LC20545E
Lab Chip, 2011,11, 4057-4062

  •   Request permissions

    Millifluidic droplet analyser for microbiology

    L. Baraban, F. Bertholle, M. L. M. Salverda, N. Bremond, P. Panizza, J. Baudry, J. A. G. M. de Visser and J. Bibette, Lab Chip, 2011, 11, 4057
    DOI: 10.1039/C1LC20545E

Search articles by author

Spotlight

Advertisements