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Issue 2, 2011
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PDMS free-flow electrophoresis chips with integrated partitioning bars for bubble segregation

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Abstract

In this work, a microfluidic free-flow electrophoresis device with a novel approach for preventing gas bubbles from entering the separation area is presented. This is achieved by integrating partitioning bars to reduce the channel depth between electrode channels and separation chamber in order to obtain electrical contact and simultaneously prevent bubbles from entering the separation area. The three-layer sandwich chip features a reusable carrier plate with integrated ports for fluidic connection combined with a softlithographically cast microfluidic PDMS layer and a sealing glass slide. This design allows for a straightforward and rapid chip prototyping process. The performance of the device is demonstrated by free-flow zone electrophoretic separations of fluorescent dye mixtures as well as by the separation of labeled amines and amino acids with separation voltages up to 297 V.

Graphical abstract: PDMS free-flow electrophoresis chips with integrated partitioning bars for bubble segregation

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Article information


Submitted
27 Aug 2010
Accepted
15 Oct 2010
First published
08 Nov 2010

Lab Chip, 2011,11, 309-314
Article type
Paper

PDMS free-flow electrophoresis chips with integrated partitioning bars for bubble segregation

S. Köhler, C. Weilbeer, S. Howitz, H. Becker, V. Beushausen and D. Belder, Lab Chip, 2011, 11, 309
DOI: 10.1039/C0LC00347F

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