Issue 14, 2011
From the journal:

Journal of Materials Chemistry

Self assembly of acetylcholinesterase on a gold nanoparticles–graphenenanosheet hybrid for organophosphate pesticide detection using polyelectrolyte as a linker†

Ying Wang,ab   Sheng Zhang,b   Dan Du,bc   Yuyan Shao,b   Zhaohui Li,b   Jun Wang,b   Mark H. Engelhard,b   Jinghong Li*a  and  Yuehe Lin*b  

* Corresponding authors

a Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, PR China
E-mail: jhli@mail.tsinghua.edu.cn

b Pacific Northwest National Laboratory, Richland, Washington, USA
E-mail: yuehe.lin@pnl.gov
Tel: +1 509-371-6241

c Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, PR China

Abstract

A nanohybrid of gold nanoparticles (Au NPs) and chemically reduced graphene oxide nanosheets (cr-Gs) was synthesized by in situgrowth of Au NPs on the surface of graphene nanosheets in the presence of poly(diallyldimethylammonium chloride) (PDDA), which not only improved the dispersion of Au NPs but also stabilized cholinesterase with high activity and loading efficiency. The obtained nanohybrid was characterized by TEM, XRD, XPS, and electrochemistry. Then an enzyme nanoassembly (AChE/Au NPs/cr-Gs) was prepared by self-assembling acetylcholinesterase (AChE) on Au NP/cr-Gs nanohybrid. An electrochemical sensor based on AChE/Au NPs/cr-Gs was further developed for ultrasensitive detection of organophosphate pesticide. The results demonstrate that the developed approach provides a promising strategy to improve the sensitivity and enzyme activity of electrochemical biosensors.

Graphical abstract: Self assembly of acetylcholinesterase on a gold nanoparticles–graphene nanosheet hybrid for organophosphate pesticide detection using polyelectrolyte as a linker

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Article information

DOI
https://doi.org/10.1039/C0JM03441J
Article type
Paper
Submitted
12 Oct 2010
Accepted
02 Feb 2011
First published
25 Feb 2011

J. Mater. Chem., 2011,21, 5319-5325

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Self assembly of acetylcholinesterase on a gold nanoparticles–graphene nanosheet hybrid for organophosphate pesticide detection using polyelectrolyte as a linker

Y. Wang, S. Zhang, D. Du, Y. Shao, Z. Li, J. Wang, M. H. Engelhard, J. Li and Y. Lin, J. Mater. Chem., 2011, 21, 5319 DOI: 10.1039/C0JM03441J

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