Issue 8, 2011

Systems biology and physical biology of clathrin-mediated endocytosis


In this review, we describe the application of experimental data and modeling of intracellular endocytic trafficking mechanisms with a focus on the process of clathrin-mediated endocytosis. A detailed parts-list for the proteinprotein interactions in clathrin-mediated endocytosis has been available for some time. However, recent experimental, theoretical, and computational tools have proved to be critical in establishing a sequence of events, cooperative dynamics, and energetics of the intracellular process. On the experimental front, total internal reflection fluorescence microscopy, photo-activated localization microscopy, and spinning-disk confocal microscopy have focused on assembly and patterning of endocytic proteins at the membrane, while on the theory front, minimal theoretical models for clathrin nucleation, biophysical models for membrane curvature and bending elasticity, as well as methods from computational structural and systems biology, have proved insightful in describing membrane topologies, curvature mechanisms, and energetics.

Graphical abstract: Systems biology and physical biology of clathrin-mediated endocytosis

Supplementary files

Article information

Article type
Review Article
18 Apr 2011
20 Jun 2011
First published
26 Jul 2011

Integr. Biol., 2011,3, 803-815