Issue 5, 2011

Sequencing transcriptomes in toto


The development of next-generation sequencing technologies has enabled the transcriptome to be measured and characterized at a level which was previously unattainable. Shot gun sequencing of RNAs, or RNA-Seq as it is known, is providing the means to simultaneously survey locus activity, transcript-specific expression, sequence content of transcripts and transcriptome discovery. This article discusses the current state of RNA-Seq, its potential for redefining transcriptomics and some of the challenges associated with this revolutionary technology.

Graphical abstract: Sequencing transcriptomes in toto

Article information

Article type
Review Article
01 Jul 2010
27 Dec 2010
First published
04 Feb 2011

Integr. Biol., 2011,3, 522-528