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Issue 1, 2011
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Performance evaluation of phycocyanin probes for the monitoring of cyanobacteria

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Abstract

The performance of two field probes (YSI 6600 and TriOS), used for the measurement of in vivophycocyanin fluorescence, was compared and validated in the laboratory in 2008 and 2009 with cultures of Microcystis aeruginosa and field samples. The background noise of the two probes was low and the detection limits were estimated at 1500 cells mL−1 for the YSI and 0.69 µg PC L−1 for the TriOS. The linearity and repeatability of both probes have been excellent. Strong relationships were observed between the in vivo fluorescence and the total cyanobacterial biovolume (R2 = 0.82 YSI; 0.83 TriOS) or the abundance (R2 = 0.71 YSI; 0.75 TriOS) of cyanobacteria. However, the difference between cell densities determined by microscopy and measured by the YSI can be very large and has been associated to the variability of cell volume among cyanobacteria. This last observation makes the YSI a qualitative tool if a post-calibration is not done. The analysis of filtrated samples showed that dissolved phycocyanin (extracellular) may represent a significant fluorescence signal. No relationship could be established between the abundance, the total cyanobacterial biovolume or the in vivo fluorescence of phycocyanin and the concentrations of cyanotoxins (R2 ≤ 0.22).

Graphical abstract: Performance evaluation of phycocyanin probes for the monitoring of cyanobacteria

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Publication details

The article was received on 20 Jul 2010, accepted on 20 Sep 2010 and first published on 26 Oct 2010


Article type: Paper
DOI: 10.1039/C0EM00366B
Citation: J. Environ. Monit., 2011,13, 110-118
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    Performance evaluation of phycocyanin probes for the monitoring of cyanobacteria

    C. Bastien, R. Cardin, É. Veilleux, C. Deblois, A. Warren and I. Laurion, J. Environ. Monit., 2011, 13, 110
    DOI: 10.1039/C0EM00366B

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