Jump to main content
Jump to site search

Issue 33, 2011
Previous Article Next Article

Experimental and theoretical study of the metastable decay of negatively charged nucleosides in the gas phase

Author affiliations

Abstract

Fragmentation of metastable anions of all deoxynucleosides and nucleosides constituting DNA and RNA has been studied experimentally and by computer simulations. The ions were formed through deprotonation in matrix assisted laser desorption/ionisation (MALDI). Clear difference in fragmentation patterns was obtained for nucleosides containing purinevs.pyrimidine bases. To elucidate the role of various potential deprotonation sites, systematic blocking by chemical modification was performed and this gave unambiguous correlation between deprotonation sites and fragments observed. Classical dynamics simulations of the fragmentation process, using density functional theory to describe the electronic degrees of freedom, were performed for the various deprotonation sites. These were found to reproduce the observed fragmentation patterns remarkably well.

Graphical abstract: Experimental and theoretical study of the metastable decay of negatively charged nucleosides in the gas phase

Back to tab navigation

Supplementary files

Article information


Submitted
23 Apr 2011
Accepted
24 Jun 2011
First published
18 Jul 2011

Phys. Chem. Chem. Phys., 2011,13, 15283-15290
Article type
Paper

Experimental and theoretical study of the metastable decay of negatively charged nucleosides in the gas phase

H. D. Flosadóttir, H. Jónsson, S. Th. Sigurdsson and O. Ingólfsson, Phys. Chem. Chem. Phys., 2011, 13, 15283
DOI: 10.1039/C1CP21298B

Social activity

Search articles by author

Spotlight

Advertisements