Issue 30, 2011
From the journal:

Physical Chemistry Chemical Physics

Protein stability in an ionic liquid milieu: on the use of differential scanning fluorimetry

João V. Rodrigues,a   Vesna Prosinecki,a   Isabel Marrucho,a   Luís Paulo N. Rebeloa  and  Cláudio M. Gomes*a  

* Corresponding authors

a Instituto Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República 127, 2780-756 Oeiras, Portugal
E-mail: gomes@itqb.unl.pt
Web: www.itqb.unl.pt

Abstract

Protein stability is a major bottleneck in the biotechnological application of ionic liquid-containing solvents, either in the frame of biocatalysis or protein storage. Herein, differential scanning fluorimetry was successfully implemented as a high throughput method to fast scan the impact of a number of cholinium-based ionic liquids on the stability of proteins.

Graphical abstract: Protein stability in an ionic liquid milieu: on the use of differential scanning fluorimetry

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Article information

DOI
https://doi.org/10.1039/C1CP21187K
Article type
Communication
Submitted
14 Apr 2011
Accepted
13 Jun 2011
First published
27 Jun 2011

Phys. Chem. Chem. Phys., 2011,13, 13614-13616

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Protein stability in an ionic liquid milieu: on the use of differential scanning fluorimetry

J. V. Rodrigues, V. Prosinecki, I. Marrucho, L. P. N. Rebelo and C. M. Gomes, Phys. Chem. Chem. Phys., 2011, 13, 13614 DOI: 10.1039/C1CP21187K

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