Issue 26, 2011
From the journal:

Chemical Communications

A luminescent oxygen channeling biosensor that measures small GTPase activation†

Björn Niebel,a   Benjamin Weiche,a   Alan L. Mueller,b   Dean Y. Li,c   Nora Karnowskia  and  Michael Famulok*a  

* Corresponding authors

a LIMES Institute, Chemical Biology & Medicinal Chemistry Unit, University of Bonn, 53121 Bonn, Germany
E-mail: m.famulok@uni-bonn.de
Fax: +49-228-735388

b Navigen Pharmaceuticals, 383 Colorow Drive, Salt Lake City, USA
E-mail: amueller@navigenpharma.com

c Program in Molecular Medicine, University of Utah, Salt Lake City, USA
E-mail: dean.li@hmbg.utah.edu

Abstract

We established a homogeneous luminescent oxygen channeling sensor for measuring activation states of small GTPases. The assay quantifies activated GTPases in cell lysates, can be applied to different GTPases, and can be used for multiplex screening. The study will provide guidelines for determining activation states of diverse GTPases in various biological contexts.

Graphical abstract: A luminescent oxygen channeling biosensor that measures small GTPase activation

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Article information

DOI
https://doi.org/10.1039/C1CC11944C
Article type
Communication
Submitted
06 Apr 2011
Accepted
16 May 2011
First published
31 May 2011

Chem. Commun., 2011,47, 7521-7523

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A luminescent oxygen channeling biosensor that measures small GTPase activation

B. Niebel, B. Weiche, A. L. Mueller, D. Y. Li, N. Karnowski and M. Famulok, Chem. Commun., 2011, 47, 7521 DOI: 10.1039/C1CC11944C

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