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Issue 26, 2011
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Engineering of a bis-chelator motif into a protein α-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy

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Abstract

Attachment of two nitrilotriacetic acid-based ligands to a protein α-helix in an i, i + 4 configuration produces an octadentate chelating motif that is able to bind paramagnetic lanthanide ions rigidly and with high affinity, leading to large pseudocontact shifts and residual dipolar couplings in the NMR spectrum.

Graphical abstract: Engineering of a bis-chelator motif into a protein α-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy

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Publication details

The article was received on 03 Apr 2011, accepted on 12 May 2011 and first published on 27 May 2011


Article type: Communication
DOI: 10.1039/C1CC11893E
Citation: Chem. Commun., 2011,47, 7368-7370
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    Engineering of a bis-chelator motif into a protein α-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy

    J. D. Swarbrick, P. Ung, X. Su, A. Maleckis, S. Chhabra, T. Huber, G. Otting and B. Graham, Chem. Commun., 2011, 47, 7368
    DOI: 10.1039/C1CC11893E

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