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Issue 14, 2011
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Bioorthogonal chemical tagging of protein cholesterylation in living cells

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Abstract

We report the first chemical probe for bioorthogonal chemical tagging of post-translationally cholesterylated proteins with an azide in living cells. This enables rapid multiplexed fluorescence detection and affinity labelling of protein cholesterylation, as exemplified by Sonic hedgehog protein, opening up new approaches for the de novo identification of cholesterylated proteins.

Graphical abstract: Bioorthogonal chemical tagging of protein cholesterylation in living cells

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Publication details

The article was received on 31 Oct 2010, accepted on 21 Dec 2010 and first published on 11 Jan 2011


Article type: Communication
DOI: 10.1039/C0CC04710D
Citation: Chem. Commun., 2011,47, 4081-4083
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    Bioorthogonal chemical tagging of protein cholesterylation in living cells

    W. P. Heal, B. Jovanovic, S. Bessin, M. H. Wright, A. I. Magee and E. W. Tate, Chem. Commun., 2011, 47, 4081
    DOI: 10.1039/C0CC04710D

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