Issue 14, 2011
From the journal:

Chemical Communications

Development of a system to sensitively and specifically visualize c-fosmRNA in living cells using bispyrene-modified RNA probes†

Reiko Waki,a   Asako Yamayoshi,a   Akio Koboria  and  Akira Murakami*a  

* Corresponding authors

a Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan
E-mail: akiram@kit.ac.jp
Fax: +81 75-7247814
Tel: +81 75-7247814

Abstract

In situ visualization of c-fosmRNA was shown both in fixed cells and in living cells using bispyrene-modified 2′-O-methyl RNA probes (OMUpy2) or phosphorothioate modified OMUpy2 (OMUpy2-S), which was RNA-specific with high sensitivity, and a system for time-lapse imaging of c-fosmRNA was successfully developed.

Graphical abstract: Development of a system to sensitively and specifically visualize c-fosmRNA in living cells using bispyrene-modified RNA probes

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Article information

DOI
https://doi.org/10.1039/C0CC04639F
Article type
Communication
Submitted
27 Oct 2010
Accepted
29 Jan 2011
First published
01 Mar 2011

Chem. Commun., 2011,47, 4204-4206

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Development of a system to sensitively and specifically visualize c-fosmRNA in living cells using bispyrene-modified RNA probes

R. Waki, A. Yamayoshi, A. Kobori and A. Murakami, Chem. Commun., 2011, 47, 4204 DOI: 10.1039/C0CC04639F

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