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Issue 12, 2011
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Fast analysis of flavonoids in apple juice on new generation halo column by SPE-HPLC

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Abstract

A fast SPE-HPLC method is described for the analysis of flavonoids in apple juice. The mobile phase used was water-acetonitrile (60 : 40, v/v) at 0.80 mL min−1 flow rate with UV detection at 252 nm. The column used was new generation Halo C18 (100 × 46 mm; shell particles; 2.7 μm). The capacity factors were in the range of 0.67 to 20.56, respectively. The separation and resolution factors ranged from 1.18–2.21 and 1.00–10.00, respectively. The values for LOD and LOQ for flavonoids ranged from 0.40–5.5 ng and 2.25–26.0 ng, respectively. The linearity was observed in the concentration ranges of 0.01 to 0.10 mg mL−1 for all flavonoids. The concentrations of quercetin, apigenin, kaemferol and chrysin in apple juice were 4.00, 0.50, 0.40 and 0.25 mg kg−1, respectively. Therefore, the reported method can be used for the quality control of apple juice in food industries. Besides, this method can also be used for the analyses of flavonoids in other fruits and vegetables.

Graphical abstract: Fast analysis of flavonoids in apple juice on new generation halo column by SPE-HPLC

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Publication details

The article was received on 12 Aug 2011, accepted on 08 Sep 2011 and first published on 19 Oct 2011


Article type: Paper
DOI: 10.1039/C1AY05496A
Citation: Anal. Methods, 2011,3, 2836-2841

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    Fast analysis of flavonoids in apple juice on new generation halo column by SPE-HPLC

    I. Ali, S. M. Z. Al-kindy, F. O. Suliman and S. D. Alam, Anal. Methods, 2011, 3, 2836
    DOI: 10.1039/C1AY05496A

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