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Issue 12, 2011
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A new capillary electrophoresis-direct chemiluminescence system for the determination of epinephrine and mechanism study

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Abstract

Epinephrine can significantly enhance the intensity of chemiluminescence (CL) produced by the reaction between luminol and potassium hexacyanoferrate(III) (K3[Fe(CN)6] under alkaline conditions, and the increased CL intensity is proportional to the concentration of epinephrine. Based on this phenomenon, a new, simple and rapid capillary electrophoresis (CE)-direct CL detection method was established for sensitive determination of epinephrine. Optimal separation and detection were obtained with an electrophoretic buffer of 20 mM sodium (pH = 9.00) borate containing 5 × 10−5 M luminol and a post-column solution of 1 × 10−4 M K3[Fe(CN)6] in 0.5 M NaOH. A limit of detection of 8.3 × 10−9 M was achieved. The relative standard derivations (RSD) of CL intensity and migration time were 3.5% and 0.8%, respectively. The applicability of the method for analysis of pharmaceutical and biological samples was examined. And the possible mechanism of the enhanced CL intensity was proposed via examining CL and UV-vis spectra.

Graphical abstract: A new capillary electrophoresis-direct chemiluminescence system for the determination of epinephrine and mechanism study

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Article information


Submitted
21 Jul 2011
Accepted
19 Sep 2011
First published
20 Oct 2011

Anal. Methods, 2011,3, 2893-2897
Article type
Paper

A new capillary electrophoresis-direct chemiluminescence system for the determination of epinephrine and mechanism study

Y. Su, C. Chen, X. Hou and J. Zhang, Anal. Methods, 2011, 3, 2893
DOI: 10.1039/C1AY05435J

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