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Issue 4, 2011
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Enzyme-luminescence method: Tool for evaluation of neuronal differentiation based on real-time monitoring of dopamine release response from PC12 cells

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Abstract

We previously developed an easy and sensitive method for real-time monitoring of dopamine release from PC12 cells using a combination of enzymatic oxidation of dopamine and luminal reaction. Here, we applied our enzyme-luminescence method for the evaluation of neuronal differentiation in PC12 cells with NGF, via real-time monitoring of dopamine release response. ATP and muscarine-stimulated dopamine release from PC12 cells was compared before and after differentiation by the luminescence peak intensity responding to agonists' injection. A clear enhancement of dopamine release ability in PC12 cells after differentiation was demonstrated. Therefore, this enhancement in luminescence peak intensity by dopamine release upon agonist stimulation may be a good marker of differentiation in PC12 cells. These results imply that the enzyme-luminescence method might be used as a promising tool for evaluation of the effects of various growth factors and chemicals on neuronal differentiation in neuronal progenitor cells.

Graphical abstract: Enzyme-luminescence method: Tool for evaluation of neuronal differentiation based on real-time monitoring of dopamine release response from PC12 cells

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Publication details

The article was received on 20 Dec 2010, accepted on 21 Jan 2011 and first published on 15 Feb 2011


Article type: Paper
DOI: 10.1039/C0AY00769B
Anal. Methods, 2011,3, 837-841

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    Enzyme-luminescence method: Tool for evaluation of neuronal differentiation based on real-time monitoring of dopamine release response from PC12 cells

    T. A. Mir, H. Shinohara and Y. Shimizu, Anal. Methods, 2011, 3, 837
    DOI: 10.1039/C0AY00769B

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