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Issue 7, 2011
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Determination of GABA, glutamate, dopamine and carbamathione in brain microdialysis samples by micellar electrokinetic chromatography and laser-induced fluorescence (MEKC-LIF)

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Abstract

A micellar electrokinetic chromatography and laser-induced fluorescence (MEKC-LIF) method based on derivatization with naphthalene-2,3-dicarboxyaldehyde (NDA) was optimized to separate and detect glutamate (Glu), gamma-aminobutyric acid (GABA), dopamine (DA) and carbamathione in brain microdialysis samples. The background electrolyte (BGE) consisted of 22.5 mmol L−1lithium tetraborate, 25 mmol L−1lithium dodecylsulfate and 10% methanol. The separation was carried out on a 65 cm × 50 μm id fused-silica capillary (50 cm effective) at +15 kV voltage with a run time of 25 min. The detection limits for GABA, Glu, DA and carbamathione were 6, 10, 5 and 10 nmol L−1, respectively. This method was used to monitor GABA, Glu, DA and carbamathione in brain microdialysis samples from the nucleus accumbens after the administration of an intra-peritoneal dose of disulfiram (200 mg kg−1) and revealed a disulfiram-induced change in GABA, Glu and DA levels. This method demonstrates a simple, rapid and accurate measurement of three amino acid neurotransmitters and carbamathione for in vivo monitoring in the brain using microdialysis sampling.

Graphical abstract: Determination of GABA, glutamate, dopamine and carbamathione in brain microdialysis samples by micellar electrokinetic chromatography and laser-induced fluorescence (MEKC-LIF)

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Article information


Submitted
03 Dec 2010
Accepted
16 Feb 2011
First published
21 Jun 2011

Anal. Methods, 2011,3, 1514-1520
Article type
Paper

Determination of GABA, glutamate, dopamine and carbamathione in brain microdialysis samples by micellar electrokinetic chromatography and laser-induced fluorescence (MEKC-LIF)

S. Kaul, M. D. Faiman and C. E. Lunte, Anal. Methods, 2011, 3, 1514
DOI: 10.1039/C0AY00734J

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