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Issue 3, 2011
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Fluorescence images combined to statistic test for fingerprinting of citrus plants after bacterial infection

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Abstract

The citrus greening (or huanglongbing) disease has caused serious problems in citrus crops around the world. An early diagnostic method to detect this malady is needed due to the rapid dissemination of Candidatus Liberibacter asiaticus (CLas) in the field. This analytical study investigated the fluorescence responses of leaves from healthy citrus plants and those inoculated with CLas by images from a stereomicroscope and also evaluated their potential for the early diagnosis of the infection caused by this bacterium. The plants were measured monthly, and the evolution of the bacteria on inoculated plants was monitored by real-time quantitative polymerase chain reaction (RT-qPCR) amplification of CLas sequences. A statistical method was used to analyse the data. The selection of variables from histograms of colours (colourgrams) of the images was optimized using a paired Student's t-test. The intensity of counts for green colours from images of fluorescence had clearly minor variations for healthy plants than diseased ones. The darker green colours were the indicators of healthy plants and the light colours for the diseased. The method of fluorescence images is novel for fingerprinting healthy and diseased plants and provides an alternative to the current method represented by PCR and visual inspection. A new, non-subjective pattern of analysis and a non-destructive method has been introduced that can minimize the time and costs of analyses.

Graphical abstract: Fluorescence images combined to statistic test for fingerprinting of citrus plants after bacterial infection

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Publication details

The article was received on 02 Sep 2010, accepted on 04 Dec 2010 and first published on 19 Jan 2011


Article type: Paper
DOI: 10.1039/C0AY00538J
Citation: Anal. Methods, 2011,3, 552-556

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    Fluorescence images combined to statistic test for fingerprinting of citrus plants after bacterial infection

    F. M. Verbi Pereira, D. M. Bastos Pereira Milori, E. R. Pereira-Filho, A. L. Venâncio, M. de Sá Tavares Russo, P. K. Martins and J. Freitas-Astúa, Anal. Methods, 2011, 3, 552
    DOI: 10.1039/C0AY00538J

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