Sensitive determination of dopamine in the presence of uric acid and ascorbic acid using TiO2nanotubes modified with Pd, Pt and Aunanoparticles

Abstract

A simple modified TiO₂ nanotubes electrode was fabricated by electrodeposition of Pd, Pt and Au nanoparticles. The TiO₂ nanotubes electrode was prepared using the anodizing method, followed by modifying Pd nanoparticles onto the tubes surface, offering a uniform conductive surface for electrodeposition of Pt and Au. The performance of the modified electrode was characterized by cyclic voltammetry and differential pulse voltammetry methods. The Au/Pt/Pd/TiO₂ NTs modified electrode represented a high sensitivity towards individual detection of dopamine as well as simultaneous detection of dopamine and uric acid using 0.1 M phosphate buffer solution (pH 7.00) as the base solution. In both case, electro-oxidation peak currents of dopamine were linearly related to accumulated concentration over a wide concentration range of 5.0 × 10⁻⁸ to 3.0 × 10⁻⁵ M. However in the same range of dopamine concentration, the sensitivity had a significant loss at Pt/Pd/TiO₂ NTs electrode, suggesting the necessity for Au nanoparticles in modified electrode. The limit of the detection was determined as 3 × 10⁻⁸ M for dopamine at signal-to-noise ratio equal to 3. Furthermore, the Au/Pt/Pd/TiO₂ NTs modified electrode was able to distinguish the oxidation response of dopamine, uric acid and ascorbic acid in mixture solution of different acidity. It was shown that the modified electrode possessed a very good reproducibility and long-term stability. The method was also successfully applied for determination of DA in human urine samples with satisfactory results.