Issue 20, 2011
From the journal:

Analyst

Localized surface plasmon resonance interfaces coated with poly[3-(pyrrolyl)carboxylic acid] for histidine-tagged peptide sensing

Fatma-Zohra Tighilt,ab   Palaniappan Subramanian,c   Naima Belhaneche-Bensemra,b   Rabah Boukherroub,c   Noureddine Gabouze,a   Sabrina Sama  and  Sabine Szunerits*c  

* Corresponding authors

a UDTS, 2, Bd Frantz-Fanon, B.P. 140 Alger-7 merveilles, Algiers, Algeria

b ENP, 10, Avenue Hassen Badi-B.P. 182, El Harrach, Algiers, Algeria

c Institut de Recherche Interdisciplinaire (IRI), CNRS USR 3078, Université de Lille1, Parc de la Haute Borne, 50 avenue de Halley, BP 70478, Villeneuve d'Ascq Cédex, France

Abstract

The paper reports on a novel localized surface plasmon resonance (LSPR) substrate architecture for the immobilization and detection of histidine-tagged peptides. The LSPR interface consists of an ITO (indium tin oxide) substrate coated with gold nanostructures. The latter are obtained by thermal deposition of a thin (2 nm thick) gold film followed by post-annealing at 500 °C. The LSPR interface was coated with poly[3-(pyrrolyl)carboxylic acid] thin films using electrochemical means. The ability of the LSPR interfaces coated with poly[3-(pyrrolyl)carboxylic acid] to chelate copper ions was investigated. Once loaded with metal ions, the modified LSPR interface was able to bind specifically to histidine-tagged peptides. The binding process was followed using LSPR.

Graphical abstract: Localized surface plasmon resonance interfaces coated with poly[3-(pyrrolyl)carboxylic acid] for histidine-tagged peptide sensing

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Article information

DOI
https://doi.org/10.1039/C1AN15535K
Article type
Paper
Submitted
28 Jun 2011
Accepted
28 Jul 2011
First published
26 Aug 2011

Analyst, 2011,136, 4211-4216

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Localized surface plasmon resonance interfaces coated with poly[3-(pyrrolyl)carboxylic acid] for histidine-tagged peptide sensing

F. Tighilt, P. Subramanian, N. Belhaneche-Bensemra, R. Boukherroub, N. Gabouze, S. Sam and S. Szunerits, Analyst, 2011, 136, 4211 DOI: 10.1039/C1AN15535K

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