Issue 20, 2011
From the journal:

Analyst

Two-dimensional difference gel electrophoresis applied for analytical proteomics: fundamentals and applications to the study of plant proteomics

Sandra Cristina Capaldi Arruda,a   Herbert de Sousa Barbosa,bc   Ricardo Antunes Azevedoa  and  Marco Aurélio Zezzi Arruda*bc  

* Corresponding authors

a Laboratory of Genetics and Biochemistry of Plants, Department of Genetics, Escola Superior de Agricultura Luiz de Queiroz, ESALQ, USP, 13400-970 Piracicaba, SP, Brazil

b Spectrometry, Sample Preparation and Mechanization Group-GEPAM, Institute of Chemistry, University of Campinas – UNICAMP, P.O. Box 6154, 13083-970 Campinas, SP, Brazil

c National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas – UNICAMP, P.O. Box 6154, 13083-970 Campinas, SP, Brazil

Abstract

The present review reports the principles, fundamentals and some applications of two-dimensional difference gel electrophoresis for analytical proteomics based on plant proteome analysis, also emphasizing some advantages of 2-D DIGE over 2-D PAGE techniques. Some fluorescent protein labeling reagents, methods of protein labeling, models of 2-D DIGE experiments, and some limitations of this technique are presented and discussed in terms of 2-D DIGE plant proteomes. Finally, some practical applications of this technique are pointed out, emphasizing its potentialities in plant proteomics.

Graphical abstract: Two-dimensional difference gel electrophoresis applied for analytical proteomics: fundamentals and applications to the study of plant proteomics

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Article information

DOI
https://doi.org/10.1039/C1AN15513J
Article type
Tutorial Review
Submitted
21 Jun 2011
Accepted
28 Jul 2011
First published
31 Aug 2011

Analyst, 2011,136, 4119-4126

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Two-dimensional difference gel electrophoresis applied for analytical proteomics: fundamentals and applications to the study of plant proteomics

S. C. C. Arruda, H. D. S. Barbosa, R. A. Azevedo and M. A. Z. Arruda, Analyst, 2011, 136, 4119 DOI: 10.1039/C1AN15513J

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