Luminescent ruthenium probe for the determination of acetyl phosphate in complex biological matrices

Abstract

The first probe for the fluorogenic determination of acetyl phosphate (AcP), (bpy)₂Ru(1,10-phenanthroline-5,6-dione dioxime) (RuPDO), was prepared and its reaction with AcP was studied in detail. The emission of the weakly luminescent RuPDO is red shifted and strongly enhanced upon reaction with AcP in the presence of metal cations like Zn²⁺ or Cu²⁺. The reaction occurs within 60 min incubation time under highly biocompatible conditions (aqueous buffer of pH 7, 37 °C). A linear dynamic range from 10 to 200 µmol L⁻¹ is observed with an LOD of AcP of 3.4 µmol L⁻¹ (for RuPDO-Zn). Other bio-phosphates studied show only weak interference. Furthermore, the applicability of the probe in complex biological matrices was evaluated.