Rational strategy of magnetic relaxation switches for glycoprotein sensing

Abstract

A rational strategy of magnetic relaxation switches was proposed here to detect a₁-acid glycoprotein (AGP), an acute phase a-globulin plasma glycoprotein. The assay was based on the relaxation time change between the aggregation of magnetic nanoparticles with concanavalin A and the redispersion with AGP, which can avoid the prozone effect and improve the detection accuracy. The assay was an easy and efficient method with two mixing steps and one measurement step, showing a detection limit of 0.66 nM in 0∼0.3 μg mL⁻¹AGP, which was far lower than its normal level in human plasma.