Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 2, 2011
Previous Article Next Article

A microfluidic array with cellular valving for single cell co-culture

Author affiliations

Abstract

We present a highly parallel microfluidic approach for contacting single cell pairs. The approach combines a differential fluidic resistance trapping method with a novel cellular valving principle for homotypic and heterotypic single cell co-culturing. Differential fluidic resistance was used for sequential single cell arraying, with the adhesion and flattening of viable cells within the microstructured environment acting to produce valves in the open state. Reversal of the flow was used for the sequential single cell arraying of the second cell type. Plasma stencilling, along the linear path of least resistance, was required to confine the cells within the trap regions. Prime flow conditions with minimal shear stress were identified for highly efficient cell arraying (∼99%) and long term cell culture. Larger trap dimensions enabled the highest levels of cell pairing (∼70%). The single cell co-cultures were in close proximity for the formation of connexon structures and the study of contact modes of communication. The research further highlights the possibility of using the natural behaviour of cells as the working principle behind responsive microfluidic elements.

Graphical abstract: A microfluidic array with cellular valving for single cell co-culture

Back to tab navigation

Supplementary files

Publication details

The article was received on 06 Jul 2010, accepted on 28 Sep 2010 and first published on 27 Oct 2010


Article type: Paper
DOI: 10.1039/C0LC00172D
Citation: Lab Chip, 2011,11, 231-237

  •   Request permissions

    A microfluidic array with cellular valving for single cell co-culture

    J. Frimat, M. Becker, Y. Chiang, U. Marggraf, D. Janasek, J. G. Hengstler, J. Franzke and J. West, Lab Chip, 2011, 11, 231
    DOI: 10.1039/C0LC00172D

Search articles by author

Spotlight

Advertisements