Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 21st October 2020 from 07:00 AM to 07:00 PM (BST).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 20, 2010
Previous Article Next Article

Controlled two-photonphotodegradation of PEG hydrogels to study and manipulate subcellular interactions on soft materials

Author affiliations

Abstract

Cell adhesion and detachment to and from the extracellular matrix (ECM) are critical regulators of cell function and fate due to the exchange of mechanical signals between the cell and its microenvironment. To study this cell mechanobiology, researchers have developed several innovative methods to investigate cell adhesion in vitro; however, most of these culture platforms are unnaturally stiff or static. To better capture the soft, dynamic nature of the ECM, we present a PEG-based hydrogel in which the context and geometry of the extracellular space can be precisely controlled in situvia two-photon induced erosion. Here, we characterize the two-photon erosion process, demonstrate its efficacy in the presence of cells, and subsequently exploit it to induce subcellular detachment from soft hydrogels. A working space was established for a range of laser powers required to induce complete erosion of the gel, and these data are plotted with model predictions. From this working space, two-photon irradiation parameters were selected for complete erosion in the presence of cells. Micron-scale features were eroded on and within a gel to demonstrate the resolution of patterning with these irradiation conditions. Lastly, two-photon irradiation was used to erode the material at the cell–gel interface to remove cell adhesion sites selectively, and cell retraction was monitored to quantify the mesenchymal stem cell (MSC) response to subcellular detachment from soft materials.

Graphical abstract: Controlled two-photon photodegradation of PEG hydrogels to study and manipulate subcellular interactions on soft materials

Back to tab navigation

Supplementary files

Article information


Submitted
29 Mar 2010
Accepted
15 Jul 2010
First published
13 Aug 2010

This article is Open Access

Soft Matter, 2010,6, 5100-5108
Article type
Paper

Controlled two-photon photodegradation of PEG hydrogels to study and manipulate subcellular interactions on soft materials

M. W. Tibbitt, A. M. Kloxin, K. U. Dyamenahalli and K. S. Anseth, Soft Matter, 2010, 6, 5100
DOI: 10.1039/C0SM00174K

Social activity

Search articles by author

Spotlight

Advertisements