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Issue 8, 2010
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Collagen-functionalised electrospun polymer fibers for bioengineering applications

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Abstract

Polymer electrospun fibers are gaining increasing importance in nanobiotechnology, due to their intrinsic three-dimensional topography and biochemical flexibility. Here we present an in-depth study of protein functionalisation for polymethylmethacrylate fibers. We compare different coating approaches for type I collagen, including physisorption and covalent binding methods relying on functional linkers. The biofunctionalised fibers are investigated by scanning electron and confocal laser scanning microscopy, wettability measurements, Fourier-transform infrared spectroscopy, and protein quantification assays. We demonstrate that the largest amount of proteins adsorbed on fibers does not determine the best performance in terms of cell attachment and proliferation in vitro, which is instead related to the type of linking and the relevant role played by adsorption of serum biomolecules on the three-dimensional nanostructures. This study is relevant for designing and engineering novel biomaterials and scaffold architectures based on electrospun nanofibers.

Graphical abstract: Collagen-functionalised electrospun polymer fibers for bioengineering applications

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Publication details

The article was received on 21 Oct 2009, accepted on 10 Dec 2009 and first published on 17 Feb 2010


Article type: Paper
DOI: 10.1039/B921932C
Citation: Soft Matter, 2010,6, 1668-1674
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    Collagen-functionalised electrospun polymer fibers for bioengineering applications

    A. Polini, S. Pagliara, R. Stabile, G. S. Netti, L. Roca, C. Prattichizzo, L. Gesualdo, R. Cingolani and D. Pisignano, Soft Matter, 2010, 6, 1668
    DOI: 10.1039/B921932C

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