Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 8, 2010
Previous Article Next Article

Collagen-functionalised electrospun polymer fibers for bioengineering applications

Author affiliations

Abstract

Polymer electrospun fibers are gaining increasing importance in nanobiotechnology, due to their intrinsic three-dimensional topography and biochemical flexibility. Here we present an in-depth study of protein functionalisation for polymethylmethacrylate fibers. We compare different coating approaches for type I collagen, including physisorption and covalent binding methods relying on functional linkers. The biofunctionalised fibers are investigated by scanning electron and confocal laser scanning microscopy, wettability measurements, Fourier-transform infrared spectroscopy, and protein quantification assays. We demonstrate that the largest amount of proteins adsorbed on fibers does not determine the best performance in terms of cell attachment and proliferation in vitro, which is instead related to the type of linking and the relevant role played by adsorption of serum biomolecules on the three-dimensional nanostructures. This study is relevant for designing and engineering novel biomaterials and scaffold architectures based on electrospun nanofibers.

Graphical abstract: Collagen-functionalised electrospun polymer fibers for bioengineering applications

Back to tab navigation

Article information


Submitted
21 Oct 2009
Accepted
10 Dec 2009
First published
17 Feb 2010

Soft Matter, 2010,6, 1668-1674
Article type
Paper

Collagen-functionalised electrospun polymer fibers for bioengineering applications

A. Polini, S. Pagliara, R. Stabile, G. S. Netti, L. Roca, C. Prattichizzo, L. Gesualdo, R. Cingolani and D. Pisignano, Soft Matter, 2010, 6, 1668
DOI: 10.1039/B921932C

Social activity

Search articles by author

Spotlight

Advertisements